Convenient histochemical phosphatase technique

Histochemical Localization of Alkaline Phosphatase in The

CHEMICAL investigations have shown that alkaline phosphatase is present in the retina (De Conciliis, 1934; Reis, 1951). In these investigations the whole retina was homogenized and the enzyme estimated in extract. Obviously the estimations of enzyme activity by such a necessarily gross method give no idea of the exact distribution of the enzyme in this, functionally, so finely differentiated ti...

A new technique for the histochemical demonstration of phosphatase in hard tissue.

Histochemical and biochemical studies on leukocyte alkaline phosphatase activity.

The histochemical demonstration of alkaline phosphatase in unfixed frozen sections.

Although, ideally, enzyme histochemistry should be performed on unfixed sections, so as to avoid potential artifacts inherent in chemical fixation, it is usually considered necessary to fix sections so as to stop the 'soluble' enzymes from becoming lost into the incubation medium. However, provided that suitable frozen sections are used, alkaline phosphatase can be demonstrated fully in unfixed...

Histochemical demonstration of acid phosphatase activity in various blood cells.

Acid phosphatase activity of various blood cells were histochemically observed on peripheral blood and bone marrow smears using the azo dye method which had been reported in my previous paper, and following results were obtained. 1) In the neutrophilic granulocyte series, the largest acid phosphatase score was obtained in immature cells, which might include promyelocytes, myelocytes, and metamy...